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Since no commercial cystine-free IMDM is available, we customized IMDM based on a DMEM medium (#D9800-28, USBiological, Salem, MA) without amino acids, glucose, sodium bicarbonate, and sodium pyruvate
The cercarial pellet was resuspended in 2 ml of cold HBSS containing 2% amphotericin B, mixed vigorously through a pipette, vortexed for 4 min to induce tail shedding, and incubated on ice for 10 min to concentrate the NTS
The anti-inflammatory properties mean less redness, fewer angry breakouts, and faster healing of existing blemishes
The fluorescence intensity was significantly enhanced by the addition of sodium ascorbate (SA) (Fig
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