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The ratio of Cys-SS n H species production was examined in the presence of 3MP
they can change the final amount dramatically
Repeated freeze-thaw cycling is one of the more common causes of peptide degradation, since the formation and melting of ice crystals can physically disrupt peptide bonds with each cycle
E.g., if the rate constant for N-acetylcysteine oxidation by H 2 O 2 is 2.3 M 1 s 1 , and the H 2 O 2 concentration is 1 M, then the half-time for NAC oxidation is 3 10 5 s, or 84 h
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