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The concentrated enzyme was loaded onto a Superdex 200 Prep Grade column equilibrated with 25 mM HEPES, 150 mM NaCl, and 20 mM cysteine at pH 7.4

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Application Glutathione reductase (GR) from bakers yeast ( Saccharomyces cerevisiae ) has been used-for quantifying the myocardial tissue glutathione content using a glutathione reductase-5,5-dithiobis (2-nitrobenzoic acid)-based enzymatic recycling assayfor the quantification of reduced glutathione (GSH) in the oocytes, using a slightly modified microglutathione assay, obtained from prepubertal giltsfor the preparation of total GSSG (glutathione disulphide) + GSH measurement, where all available GSSG was reduced to GSH, in rat lensfor the quantification of intracellular reduced glutathione (GSH) in the oocytes obtained from rats Glutathione Reductase (GR) from bakers yeast has been used:in the glutathione assay to determine glutathione concentration.as a standard in the generation of calibration curve.as an antigen to measure plasma activity of GR