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Proof of principle has been shown in experimental models: e.g
64,65 Tumors may also evade immune surveillance by downregulating tumor-associated antigens (TAAs), selecting for neoantigen-depleted clones, or upregulating alternative inhibitory checkpoints, as observed in EGFR-mutant non-small cell lung cancer (NSCLC) following targeted therapy
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The 20-gauge needle used to add bacteriostatic water is too large for subcutaneous injection and causes unnecessary tissue trauma
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