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Effect on cell viability was assessed 72 h after transfection with GSTO1 siRNA or siSCRAM by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and normalized to mock-transfected control (transfection reagent)
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Severe behavioral impairment and neuronal death of mice given the ferroptosis inhibitor ferrostatin-1 24 h before 1-methyl- 4-phenyl-1, 2, 3, 6-tetrahydropyridine(MPTP) were greatly reversed [86]
Tumours were induced, and upon presentation of clinical symptoms of tumour burden, animals were randomized and treated with 150 mg kg 1 5FU three times per week, with 250 mg kg 1 metformin or vehicle control delivered concurrently (Fig
& Ghigo, D
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