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Serial dilutions of unfused albumin variants (0.034375.2 nM) or GLP-1 albumin fusions (0.031368.5 nM) (100 L/well), diluted in PBS/T/M (pH 5.5 or pH 7.4), were added for 1 h at room temperature
The paper closed with a careful prediction: there likely existed an endogenous mammalian analog to the exendin peptides. He was describing GLP-1 without knowing it had already been found
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Exendin-4 inhibits glioma cell migration, invasion and epithelial-to-mesenchymal transition through GLP-1R/sirt3 pathway
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