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Then, cells were trypsinized and 2 10 6 cells incubated with 0.5 mmol L 1 of the spin probe CMH (1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine), which detects intracellular and extracellular superoxide ion, peroxyl radical, peroxynitrite, and nitrogen dioxide 28

36 h), Toremifene and 4-OHT

Because the molybdenum content of plants depends on the soil molybdenum content and other environmental conditions, the molybdenum content of foods can vary considerably (51, 57)

Primary antibodies were detected with anti-rat HRP-conjugated polymer (Biocare, cat# BRR4016H), 3,3-diaminobenzidine (DAB) substrate (provided in the Leica BOND Polymer Refine Detection System, cat# DS9800), and for 4-HNE staining Bond DAB Enhancer (Leica Biosystems, cat# AR9432)

Werner and Le Roith, 2000