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Despite decades of lifestyle-based interventions, long-term weight loss has proven elusive for many, driving an urgent need for safe and effective medical therapies

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For example, a patient with a BMI of 31 may technically meet BMI requirements, but insurance approval could still be denied if required documentation is missing

Patients should monitor blood glucose closely and work with healthcare providers to titrate diabetes medications appropriately, often requiring proactive insulin or sulfonylurea dose reductions

The supernatant was incubated with nickel Sepharose resin (GE healthcare) plus 15 mM imidazole at 4 C overnight