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I can empathise with this, and experienced this first-hand- but also acknowledge as a current educator, that there are limitations in what can be covered in an undergraduate degree
Neural organoids also lack vascularization, limiting the nutrient and metabolite exchange from the innermost regions of these structures, resulting in cell death in those regions (Lancaster and Knoblich, 2014)
How fast you inject the water matters
This mix might make it more effective
For twice-daily protocols, aim for a 10-12 hour separation (e.g., 8 AM and 8 PM)
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