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Acute cardiotoxicity may emerge as acute and usually reversible heart failure and/or acute arrhythmogenicity which usually manifests as premature ventricular beats observed after 10 min to 24 h following infusion of DOX ( Regarding chronic anthracyclines cardiotoxicity, a large study from the year 2003 revealed that 5%, 16%, and 26% of patients experienced DOX-related heart failure at cumulative doses of 400, 500, and 550 mg/m 2 respectively

Animal Model and Ethics Statement Wistar rats from the breeding colony at the Universidade do Extremo Sul Catarinense (UNESC), Brazil, were housed four times per cage under controlled conditions of temperature (22 1 C), relative humidity (4555%) and light-dark cycle (12:12 p.m., light on at 6 a.m.)

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Typical treatment cycles run 2 to 4 weeks for acute injuries and can be extended or repeated for chronic conditions