A well-documented COA would normally identify the product and lot and report the applicable test items, specifications, results, methods or method references, and authorisation information
Afterwards, cells were blocked, permeabilized and incubated with a primary mouse monoclonal antibody to H2AX (Ser-139) (Upstate Biotechnology, Lake Placid, NY, USA) diluted 1:500 for 16 h at 4 C, followed by incubation with anti-mouse FITC secondary antibody (Sigma-Aldrich) diluted 1:200 for 2 h at room temperature, or with a mouse anti-active caspase 3 (BD, Pharmigen, San Diego, CA, USA) diluted 1:50 for 16 h at 4 C
Cell viability and Melanin quantification assay Normal human epidermal melanocytes (Cascade Biologics TM (Portland, OR)) were cultured in HMGS (Cascade Biologics) supplemented Medium 254
Content per 1 capsule: 350 mg liposomal glutathione
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