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Finally, the sections were placed in PBS at 8 C until quantification was performed

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It is not intended for human or animal consumption, medical treatment, or diagnostic purposes

A longer supplementation period and larger sample size, may perhaps allow improved detection of mTOR phosphorylation

The endothelium, the inner lining of blood vessels, plays a vital role in maintaining vascular homeostasis by regulating vasodilation, blood flow, and platelet aggregation [65]