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Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample

Enzyme-linked immunosorbent assay (ELISA) Equal amounts of kidneys from each group were homogenized in lysate, and inflammatory cytokines, includingTNF-, IL-1, IL-6 were measured in the lysed supernatant using ELISA kits according to the manufacturers instructions (Thermo Fisher Scientific, United States)

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Benefits of IV drip therapy IV drips therapy gives you a rapid energy and nutritional boost

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