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Ethics statement The animal study was approved by Shanghai Ocean University Animal Ethics Committee

All animals were housed at standard temperature (~22C) and humidity-controlled conditions under a 14L:10D photoperiod and provided ad libitum access to water and a low-fat-purified diet upon arrival (10% calories from fat D12450H Research Diets Inc)

Product Specifications Laboratory Handling Store lyophilised vials at -20C in a light-protected environment

What if I am switching from a pre-filled pen to a compounded vial

Throughout the study, the mice had free access to standard laboratory granular diets and distilled water