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What Is So Specific About Vitamin B12
Its mainly these forms that lead to high B12 blood levels
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Null mutations were made using the lambda red recombinase method to replace the open reading frame (Gebendorfer et al., 2012) with a chloramphenicol resistance cassette amplified from the pKD3 template (Datsenko and Wanner, 2000)
The products we worked with had the old formulation, but the following sections detail the new one
The fixed tissue was washed twice for 10 minutes in 0.05 M phosphate-buffered saline (PBS)
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