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canettii C59 was achieved using the BAC-recombineering (BAC-rec) strategy 53

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use a 1 mL syringe Vial setup 1,000 mg + 5 mL Concentration 200 mg/mL Selected amount 25 mg Draw volume 0.125 mL U-100 units 12.5 units Syringe note Fits a 0.5 mL syringe Vial setup 1,000 mg + 5 mL Concentration 200 mg/mL Selected amount 50 mg Draw volume 0.25 mL U-100 units 25 units Syringe note Fits a 0.5 mL syringe Vial setup 1,000 mg + 5 mL Concentration 200 mg/mL Selected amount 75 mg Draw volume 0.375 mL U-100 units 37.5 units Syringe note Fits a 0.5 mL syringe Vial setup 1,000 mg + 5 mL Concentration 200 mg/mL Selected amount 100 mg Draw volume 0.50 mL U-100 units 50 units Syringe note Fits a 0.5 mL syringe Confirm the actual final mixed volume before using any concentration or syringe-unit calculation

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