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Method development was carried out by using a zorbax SB C18 (150 4.6 mm), 5 m column thermostated at 30C with gradient program, mobile phase with Pump A: formic acid (acetonitrile: methanol) 0.1% (80:20) (v/v/v) and Buffer I: (formic acid in water (1%) (v/v))
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Remember, the goal is to find the most effective pain management strategy with the least risk of adverse effects
Menopause brings profound changes to metabolism: estrogen decline accelerates fat accumulation around the midsection, and hunger signals intensify
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