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Total RNA was extracted from isolated islets and analyzed by rt-PCR to confirm the purity of the islets by the absence of amylase and CK19 at the transcriptional level

Both sites are equally effective for absorbing the medication, but personal preference varies

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Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min