Acquisition of 1 H-NMR spectra and metabolic profiling The lyophilized metabolic extracts were suspended in 0.6 ml of deuterated water (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing phosphate buffer (100 mM) and TSP (3-3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or TMSP- d 4 , Cambridge Isotope Laboratories) (0.5 mM) and inserted into a 5 mm NMR tube for immediate acquisition
doi: 10.1007/s40618-021-01704-3
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[5] [6] Methylcobalamin is available as an unlicensed supplement but is not a routinely recommended medicinal product in the UK and should not be substituted for licensed preparations without clinical advice
Other potential challenges include anatomical barriers of the anterior eye that limit glutathione delivery to the lens and intracellular barriers within the lens that limit glutathione delivery to different lens regions