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For example, in bone marrow-derived macrophage (BMDM), high-resolution confocal fluorescence microscopy with LC-MS/MS-targeted metabolomics reveals that upon stimulation by pathogens such as Salmonella typhimurium, the lysosomal biogenesis factor (TFEB) is activated and translocates to the nucleus to upregulate the transcription of aconitate decarboxylase 1 (Irg1/Acod1), thereby driving the massive synthesis of itaconate within mitochondria
Journal of Physiology-Paris
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