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The column was then washed with 10 CV of 25 mM imidazole (Sigma-Aldrich) in DPBS (pH 7.2), followed by elution with 10 CV of 250 mM imidazole in DPBS (pH 7.3)

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There is some evidence that high-intensity interval treatment might be comparable to moderate-intensity continuous training for reducing hepatic steatosis, but a clear recommendation cannot be made