Senescence-associated -galactosidase (SA--gal) staining SA--gal staining was performed according to the manufacturers instructions (#9860, Cell Signaling Technology)
By activating both GIP and GLP-1 receptors, tirzepatide enhances glucose-dependent insulin secretion from pancreatic beta cells, suppresses inappropriate glucagon release, and slows gastric emptying
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Furthermore, it is proposed that leptin might be more involved in elevating energy expenditure rather than merely decreasing food consumption in DEX-induced anorexia (Won Jahng et al., 2008)
And as long as it's continued to completion, I don't think efficacy is really a question at this point either. However, lower dosing often means a longer treatment course