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Samples were separated via SDS-PAGE using 10% or 12.5% acrylamide gels and, in a semi-dry western blot, transferred to either nitrocellulose (GE Healthcare) or PVDF (GE Healthcare) membranes
PCR was performed using PCR Master Mix kit (Fermentas, USA) in a final volume of 50 l
ROS at the physiological level can act as redox messengers involved in the regulation of various cellular activities, such as cell growth, differentiation, proliferation, and apoptosis (123)
Pancreatitis (rare): Acute pancreatitis has been reported with GLP-1 agonists
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