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The pellet was dissolved in 90 ml Buffer B supplemented with 500 mM KCl and 5 mM Imidazole and incubated with 2 ml TALON resin (Takara, Cat#635502) for 1 h on a rotary mixer
Call Jennifer Jose-Cappola, MD at (475) 685-3369 to ask if glutathione IV therapy is right for you
A 10 mg vial with 2 mL of bacteriostatic water gives 5 mg/mL, which puts a 2.5 mg dose at 50 units on a U-100 syringe
doi: 10.1016/j.ejphar.2007.05.072
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