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Under the stimulation of oxidative stress, keratinocytes are induced to produce CXC chemokine ligand (CXCL) 16, which further recruits CXCR6 + CD8 + T cells and the infiltration of such T cells in the skin is followed by the loss of melanocytes in vitiligo lesions (Li et al., 2017)

Column washes and elution fractions are collected in predesignated tubes for easy location of purified protein without needing to pool fraction tubes