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For MIA mice, a continuous ICV infusion of 2.5 g per day was administered over a seven-day period

Silver from the amalgam leeches out of the embedded particles and stains (ie, tattoos) selected components of the fibrous connective tissue (eg, elastic, reticulin, oxytalan fibers) and highlights the blood vessels

Moreover, elevated insulin levels resulting from hyperinsulinemia trigger insulin-like growth factor (IGF) signaling, activating key pathways such as phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) and mitogen-activated protein kinase (MAPK) [26]

Metabolism (2016) 65:1196207