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Kulak MV, Cyr AR, Woodfield GW et al (2013) Transcriptional regulation of the GPX1 gene by TFAP2C and aberrant CpG methylation in human breast cancer
The adducts were screened by the sitting-drop vapour diffusion method using commercially available screens from Molecular Dimensions and Qiagen, dispensed by the Mosquito robot (TTP Labtech)
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Since RNF4 is an E3 ubiquitin ligase possibly ubiquitinating VHL or CRBN after PROTAC addition, we also tested if the synthesized PROTACs induced CRBN or VHL degradation
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