The capillary voltage and capillary temperature were set to 3.5 KV and 320 C, respectively
Transwell assay Transwell migration assay: the Transwell chamber was coated with 50 L Matrigel (354234, BD Biosciences, Franklin Lakes, NJ, USA), and solidified at 37 C for 30 min
Potential Side Effects L-Glutamine is generally safe when taken at recommended doses (5-15 grams daily)
TEER measures the net flux of all ions (cations and anions) across the epithelium and reflects the contribution of the paracellular resistance (R para ) that reflects the resistance of the TJs, the transcellular resistance (R trans ) that reflects the resistance to ions of the apical (R api ) and basolateral (R bas ) membranes and finally the sub-epithelial resistance (R sub )
Moreover, agrin-treated pericytes exhibited elevated levels of ROS and Fe 2+ , along with reduced expression of GPX4 and FTH1, thereby promoting ferroptosis, suppressing pericyte proliferation, and compromising blood-brain barrier function