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Alzheimer's disease and neuroinflammation: will new drugs in clinical trials pave the way to a multi-target therapy
g) optionally, purifying semapeptide, wherein semapeptide amino acid sequence is Glu-Gly-Thr- Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-A present invention involves fermentation process for increase in accumulation of expressed semapeptide by inducing transformant prokaryotic cells in culture medium with chemical agent
The dialyzed polymer was subsequently lyophilized and reconstituted in sterile phosphate-buffered saline (PBS) to a final concentration of 60 mg mL for use in hydrogel formulations
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