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Although rigorous statistical methods, including multivariable regression and stratified analyses, were employed to enhance the reliability and robustness of the results, the study design does not allow us to determine whether altered vitamin B12 levels directly influence testosterone concentrations or whether hormonal changes affect vitamin B12 metabolism

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3.5 Myosin light chain kinase and cytoskeletal regulation Myosin light chain kinase (MLCK)mediated regulation of epithelial barrier function is primarily controlled by coordinated Ca 2+ /calmodulin and RhoA/Rho-associated kinase (ROCK) signalling pathways that converge on phosphorylation of the regulatory myosin light chain (MLC-2), a central determinant of actomyosin contractility and TJ permeability (Cunningham and Turner, 2012)