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coli ) strain BL21, used to express Nb35, was cultured in Luria-Bertani (LB) liquid medium (10 g tryptone, 10 g NaCL and 5 g yeast extract per litre) with continuous shaking (180 rpm) or on LB agar plate (LB medium with 15 g agar per litre) at 37C
Red flags such as rapid growth, fixation, systemic symptoms, or airway compromise require prompt evaluation
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The authors also note that future work will need safer, more refined MRS2 modulators suitable for human studies
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