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Description

Persistent activity also extends the inflammatory phase and disrupts tissue regeneration and scarring, worsening DFUs chronicity and non-healing characteristics 98

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The YAK1 -KD (kinase dead, p.K398R) and MCK1 -KD (p.K68R) mutants were created by site-directed mutagenesis (QuickChange Lightning, Agilent technologies, Santa Clara, CA, USA) according to the manufacturer instructions using primers listed in Supplementary Table 2

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The Folin phenol reagent binds to the protein, resulting in a gradual reduction, indicated by a color change from yellow to blue

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