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Topical anti-aging effects are biologically plausible because collagen, glycosaminoglycans, skin elasticity, fibroblasts, and extracellular matrix remodeling are all directly relevant to skin aging [2], [4], [12]

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investigated rapamycin pharmacokinetics following a single oral dose of 0.1 mg/kg or a five-day course of this treatment.[51] In the single-dose protocol, rapamycin concentration peaked twice, at two- and six-hours post-administration, but this pattern was not noted when the dogs were treated on five consecutive days

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