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B vitamins and vitamin C are cofactors that support enzymatic pathways but don't independently mobilize fat

We therefore measured G protein-dependent cAMP generation and -arrestin-1 and -2 recruitment with exendin-4, exendin-phe1, and exendin-asp3 at two time-points to avoid kinetic artefacts 38 , and calculated the pathway bias by fitting the response data to a modified form of the operational model of agonism 39 (Fig