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snRNA-seq and analysis For isolation of nuclei from gWAT, fresh adipose tissue was minced in nucleus preparation buffer (10 mM HEPES, 1.5 mM MgCl 2 , 10 mM KCl, 250 mM sucrose, 0.001% NP-40) with 0.2 U l Roche Protector RNase inhibitor followed by dounce homogenization

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They are often incorporated into comprehensive anti-aging and optimization protocols

Aside from this single case report, a RCT on the effect of a broccoli sprouts extract administration in 45 patients for 84 days demonstrated no variations in TSH, FT4, anti-TPO and anti-Tg autoantibodies positivity rate [158]

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