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After the treatment, samples were precipitated with MeOH and after evaporation, obtained residue was dissolved in 50 L of 50 mM HEPES buffer, diluted with 20% ACN (containing 0.1% FA and mixture of internal standards) and processed for LC-MS/MS analysis

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Drinking 1620 ounces of water 30 minutes before your workout and maintaining steady fluid intake throughout exercise becomes even more important when using pre-workout supplements alongside GLP-1 medications

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