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If you want the underlying logic explained from scratch, see the peptide reconstitution guide, the guide to how much bacteriostatic water to use, and the insulin syringe units guide
alexander12 March 7, 2019, 12:42pm 3 Sorry guess I didnt make myself clear
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If youre going to use the beads again, but not for the same exact protein, then you will want to clean and regenerate your beads to make sure that you clean out any residual proteins from your previous purification(s)