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Protein samples (20 g) were separated on NuPage Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes

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This research was supported by grants from the National Natural Science Foundation of China (32388101 and 32250001 to D.X., 32425011 and 32488302 to J.Y.), National Key R&D Program of China (2022YFD1400800 and 2021YFA1300400 to D.X.), China Postdoctoral Science Foundation (2020M670286 to Y.Y.), Postdoctoral Fellowship of Tsinghua-Peking Joint Center for Life Sciences

The most important features of PCOS are hyperandrogenism and insulin resistance, which are associated with decreased total serum carnitine levels

GHK-Cu cannot regenerate follicles that no longer exist