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The tissues were analyzed on the Ventana Discovery Ultra automation system (Ventana Medical Systems) for GLP-1 receptor expression using rabbit-anti-mGLP1R (Abcam, ab218532, lot: GR3231665-2) at a concentration of 2.7 g ml 1
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(2025) 25(02):e3027
Comparison of treatments was done on a within-subject level (each mouse was its own control) and at least 1 week elapsed between each testing condition (drug or control treatment)
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