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We exposed cell lines that had either Keap1, Nrf2 or Srxn1 tagged with green fluorescent protein (GFP) to a concentration range of DEM and monitored the response of these Nrf2 pathway components using high throughput confocal imaging (Fig

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Flow cytometry-based examination of the ferrous iron levels in 5-FU-derived persister tumors revealed increased ferrous iron staining in these residual tumors ( Figure 5D )