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One microliter of the diluted reaction was then injected onto a Waters BioAccord LC-TOF (ACQUITY I-Class UPLC and RDa detector with an ESI source) with an ACQUITY UPLC Protein BEH C4 column (300 , 1.7 m, 2.1 50 mm) at 80 C for the entirety of the analysis

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This localised action allows for a fine-tuned regulation of different brain functions related to appetite

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