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Its broad hedge is not a hedge across things it is inventing
The resulting capped -Glu-FA residue was submitted to CGenFF 40,41,42 to retrieve topology and parameter files
J Clin Invest 2014
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The supernatant was then loaded on top of a 12 mL 1050% sucrose gradient (25 mM HEPES pH 7.5, 5 mM MgCl 2 , 0.1 M KCl, 2 mM DTT, 0.1 mg/mL cycloheximide) and spun in an ultracentrifuge at 35,000 RPM at 4 C for 2 h
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