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Multiple injectable peptidic GLP-1R agonists are approved for the treatment of type 2 diabetes mellitus (T2DM), including liraglutide and semaglutide, which are also approved for the treatment of obesity
Water restriction has been demonstrated to rapidly reduce bowel movements in healthy people (Klauser 1990), and low fluid consumption has been associated with an increased risk of chronic constipation (Markland 2013)
Then the brains and serum were collected (see ESM Methods: Immunohistochemistry) for analysis of secondary outcomes (stroke volume, number of neurons positive for the neuronal nuclear marker [NeuN] and serum IGF-1 levels, see ESM Methods: Ischaemic stroke volume assessment and ESM Methods: ELISA immunoassays)
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