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It expands the Context of Use of in vivo studies from "does the animal lose weight?" to "how does feeding behaviour change over 24-hour cycles, when does activity compensation occur, and what is the temporal relationship between metabolic and behavioural endpoints?" The Adverse Outcome Pathway Framework: Connecting the Dots The Adverse Outcome Pathway (AOP) framework offers a structured way to think about Context of Use across model types

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Synthetic -MSH analog developed to probe melanocortin receptor biology (pigmentation, energy, sexual behavior)