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with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)
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Este sistema funciona de la siguiente manera: El glutatin reducido ( GSH ) neutraliza radicales libres Durante este proceso se convierte en glutatin oxidado (GSSG) La enzima glutatin reductasa lo regenera nuevamente a GSH Gracias a este ciclo, el glutatin puede neutralizar mltiples ROS y proteger las estructuras celulares frente al dao oxidativo
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