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Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40
The peptide is absorbed through the thin mucous membranes under the tongue directly into the bloodstream via the sublingual veins
It is used to probe metabolic-enzyme activity
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