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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

Environmental chemical-induced intestinal microbiome alternations might lead to systemic effects in the host ( TCE exposure, which is known to induce/exacerbate SLE in both experimental animals and humans, is also reported to cause alterations in the gut microbiome with increased abundance of genus Bifidobacterium and bacterial family Enterobacteriaceae as well as lower abundance of the genus Bacteroides and Lactobacillus in MRL+/+ mice at a high but occupationally relevant TCE dose compared to controls (105)

Some GLP-1 agonists, like semaglutide and high-dose liraglutide, are also FDA-approved for treating obesity

GLP-1 medications, like Ozempic, have become popular for their ability to support long-term weight loss when used under proper medical supervision

You can see someone who has an abnormal BMI, but can be very healthy, Dr