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Data-dependent MS2 fragmentation for compound identification and annotation was performed via the AquireX workflow (Thermo Scientific), which comprised three deep scans with MS1 resolution at 60,000 and MS2 resolution at 15,000

This is because its packed with basic amino acids like Arginine (Arg), Lysine (Lys), and Histidine (His)

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134 Here, we specifically focus on the understanding of GLP-1RA in another critical metabolic disorder: obesity

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