Prior to transfer, stain-free gels were imaged on a Biorad Chemidoc MP Imaging System (Biorad, Hercules, CA) to confirm equal protein load, and were then wet transferred onto PVDF membranes at 100 V constant for 1 h and equal transfer was routinely confirmed by Ponceau S stain
C6 cells were transfected at 80% confluence with a combination of GluN1 and GluN2A or GluN2B (3.75 g cDNA/wells) + 10 l of Lipofectamine (Invitrogen) diluted in Opti-MEM TM (Gibco TM )
Limp score Stair use Jumping confidence Play tolerance Appetite Sleep quality Stiffness after rest Stool quality if gut recovery is part of the picture This second chart is also illustrative
As the master antioxidant, glutathione helps protect cells from oxidative stress by neutralizing free radicals and regenerating other antioxidants such as vitamins C and E.* Detoxification Pathway Support Glutathione plays a central role in Phase II detoxification by conjugating with toxins and harmful substances, making them water-soluble for elimination from the body.* Cellular and Immune Health Glutathione supports optimal cellular function and repair by maintaining redox balance
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