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Additionally, the complex in vivo environment in humans is difficult to replicate in laboratory settings

Injection site can still change the first few minutes of exposure because the area around the needle sees the highest concentration before dilution (subcutaneous absorption, injection-site PK)

doi.org/10.1038/nature03000 Dalmasso, G., et al

Technical issues : If you struggle with using the injection pen or feel uncertain about any step, ask for a demonstration or further instructions

Conversely, replacement of abnormal synovial fluid with synthetic hyaluronan (also called hyaluronic acid) derivatives has been shown to reduce pain and inflammation in the arthritic knee